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South African Scientists Uncover Rare Compounds in Cannabis Leaves

Rare Compounds in Cannabis

For too long, the conversation around cannabis in South Africa has been narrowly confined, often overshadowed by debates on recreational use or the well-known psychoactive components. Yet, beneath this limited perception lies a world of intricate chemistry, brimming with therapeutic potential that is only just beginning to be unveiled. Right here, on our own soil, groundbreaking research from Stellenbosch University is challenging these outdated narratives, positioning South Africa at the forefront of global cannabis science.

This pioneering study, published in the prestigious Journal of Chromatography A, goes beyond the familiar cannabinoids like THC and CBD. It delves deep into the often-overlooked parts of the cannabis plant – particularly its leaves and inflorescence – to uncover a treasure chest of complex, health-boosting compounds. The findings are nothing short of astonishing, as local scientists have not only extensively characterized previously understudied “phenolics” but have also provided the first-ever evidence of a rare and chemically intriguing class of compounds: flavoalkaloids, in Cannabis sativa L.

This monumental work, led by Magriet Muller and Professor André de Villiers from the Department of Chemistry and Polymer Science at Stellenbosch, utilized state-of-the-art analytical techniques to dissect the chemical makeup of three commercial cannabis strains cultivated in South Africa: ‘Cape Cookie’, ‘CBG’, and ‘Blue Sky’, sourced from Cannsun Medicinals. It’s a testament to the ingenuity and scientific rigour thriving in our nation, offering a glimpse into a future where cannabis is recognized for its full spectrum of medicinal and wellness applications.

The Groundbreaking Discovery: Flavoalkaloids Make Their Debut in Cannabis

The most electrifying revelation from this Stellenbosch study is the unequivocal evidence of flavoalkaloids in the Cannabis sativa L. plant for the first time in scientific history. For many, the term “flavoalkaloid” might sound unfamiliar, and for good reason: these compounds are relatively rare in the plant kingdom, making their discovery in a species as widely studied as cannabis a true scientific breakthrough.

So, what exactly are flavoalkaloids? Imagine a powerful fusion of two distinct classes of plant compounds, each with its own significant biological activities:

  1. Flavonoids: These are well-known plant pigments and secondary metabolites found in fruits, vegetables, and many herbs. They are celebrated for their potent antioxidant, anti-inflammatory, and neuroprotective properties.
  2. Alkaloids: These are nitrogen-containing organic compounds, often found in plants, that can have profound physiological effects. Many pharmaceutical drugs, such as caffeine and morphine, are alkaloids.

A flavoalkaloid, therefore, is a hybrid molecule where an alkaloid moiety is directly linked to a flavonoid backbone. This unique structural combination suggests the potential for novel and diverse biological activities, potentially offering a broader range of therapeutic effects than either component on its own. The Stellenbosch research tentatively identified 16 flavone derivatives belonging to four distinct classes of flavoalkaloids, primarily in the leaf extracts of the ‘Blue Sky’ cannabis strain. This specificity is crucial, indicating that the therapeutic profile of cannabis can vary significantly not only between strains but also across different parts of the plant.

rare compound in cannabis
Dr Magriet Muller in front of a high-resolution mass spectrometer at the LC-MS laboratory in Stellenbosch University’s Central Analytical Facility, where part of the practical work was conducted. Credit: Wiida Fourie-Basson

Why is this discovery so profoundly significant?

  • Uncharted Chemical Territory: Finding an entirely new class of compounds in cannabis opens up an uncharted frontier in phytochemistry. It implies that despite extensive global research, we are only just scratching the surface of Cannabis sativa’s true chemical complexity.
  • Promise for Novel Therapies: In other plant species, flavoalkaloids have been shown to exhibit a wide array of bioactivities, including anti-cancer, anti-inflammatory, antimicrobial, and neuroprotective effects. The identification of these compounds in cannabis offers exciting prospects for developing entirely new therapeutic agents, potentially leading to cannabis-derived medicines with unique mechanisms of action. This moves beyond the current focus on cannabinoids and terpenes, expanding the plant’s medicinal potential exponentially.
  • Reimagining Plant Utilisation: The detection of flavoalkaloids predominantly in the leaves of a specific strain suggests that cannabis leaves, often considered by-products in some cultivation practices, may hold significant untapped value. This could lead to more sustainable and economically viable cultivation models, where the entire plant biomass is utilised for its full chemical potential, benefiting local farmers and industries in South Africa.
  • Understanding the “Entourage Effect” Further: This discovery adds another layer of complexity to the “entourage effect,” the hypothesis that various compounds in cannabis work synergistically to enhance therapeutic outcomes. The presence of flavoalkaloids suggests a richer interplay of compounds than previously understood, potentially leading to more effective, whole-plant-based remedies.

This unprecedented finding by the Stellenbosch team is a testament to the power of advanced analytical chemistry and underscores the vast, unexplored medicinal potential within Cannabis sativa L.

Diving Deeper: The Rich Tapestry of Cannabis Phenolics

Beyond the groundbreaking discovery of flavoalkaloids, the Stellenbosch study performed an extensive characterisation of other phenolic compounds in the cannabis plant. Phenolics are a diverse group of plant metabolites widely known for their crucial roles in plant defence and their beneficial effects on human health, primarily through their antioxidant and anti-inflammatory properties. Their presence is a key component of the “entourage effect,” where these compounds interact with cannabinoids and terpenes to modulate and enhance therapeutic benefits.

The research identified a remarkable total of 79 phenolic compounds, with an impressive 25 of these being reported in Cannabis sativa L. for the very first time. This expanded inventory paints a much more detailed picture of cannabis’s non-cannabinoid chemical profile. These compounds can be broadly categorised as follows:

  • Flavones and Their Glycosides: The study detected key flavones such as luteolin, apigenin, chrysoeriol, and acacetin, many of which were present as O-glucuronide or O-diglycoside derivatives. These are common flavonoids, but their specific glycosylation patterns (attachment to sugar molecules) can influence their bioavailability and biological activity.
    • Strain-Specific Variability: A particularly interesting finding was the significant difference in flavone profiles between the strains. ‘Blue Sky’ (Strain C) exhibited markedly higher levels of C-glycosylated flavones (e.g., orientin, vitexin, cytisoside) and their O-glycosylated derivatives, which were either absent or in much lower concentrations in ‘Cape Cookie’ (Strain A) and ‘CBG’ (Strain B). This underscores the importance of genotype in determining the unique chemical signature of each cannabis variety. Conversely, strains A and B showed higher levels of acacetin O-diglycosides.
  • Flavonols and Their Glycosides: The researchers also tentatively identified flavonols like quercetin and kaempferol derivatives, including a quercetin O-hexosyl O-glucuronide derivative. These compounds are potent antioxidants and contribute to the plant’s overall protective qualities.
  • Phenolic Amides and Hydroxycinnamic Acid Derivatives: The study found a range of these compounds, including N-trans-coumaroyltyramine and N-trans-feruloyltyramine, along with various derivatives of coumaric acid and caffeic acid. These compounds are known for their antioxidant properties and have been investigated for roles in anti-inflammatory processes.
  • Novel Phenolic Acid Sulfates: In another significant first, two isomeric caffeic acid sulfates were tentatively identified exclusively in the inflorescence of the ‘Blue Sky’ strain. This discovery of sulfur-containing phenolics in cannabis represents a new frontier for research into their potential bioactivities and functions within the plant.

The comprehensive nature of this phenolic characterisation highlights the extraordinary chemical diversity within Cannabis sativa L. It reinforces that different strains and even different parts of the same plant possess unique chemical profiles, suggesting that targeted cultivation and processing could yield specialized extracts for specific health conditions. The contour plots generated by the analytical method provided a rapid visual comparison of these complex profiles, making these subtle yet significant differences immediately apparent to researchers.

rare compounds in cannabis

The Cutting-Edge Toolkit: Unravelling Complexity with Advanced Analytical Chemistry

The groundbreaking discoveries from Stellenbosch University were made possible by employing a highly sophisticated and optimised analytical methodology: Comprehensive Two-Dimensional Liquid Chromatography hyphenated to High-Resolution Mass Spectrometry (HILIC × RP-LC-HR-MS). This advanced technique is crucial for dissecting the immense chemical complexity of the cannabis plant, where hundreds of compounds, many structurally similar, coexist across a wide range of concentrations.

Here’s a detailed look at the methodology that enabled these profound insights:

  1. Sample Preparation: Isolating the Targets
    To specifically target polar phenolic compounds and avoid interference from well-known apolar compounds like cannabinoids and chlorophyll, a precise sample preparation strategy was crucial:
  • Freezing and Grinding: Plant samples (inflorescence and leaves) were snap-frozen with liquid nitrogen and finely ground using a mortar and pestle. This step preserves the integrity of the compounds and maximizes extraction efficiency.
  • Defatting with Hexane: The ground material was defatted three times with hexane through sonication and centrifugation. This process effectively removed non-polar cannabinoids, chlorophyll, and other lipids, ensuring that the subsequent analysis focused on the more polar phenolic compounds.
  • Extraction with Aqueous Acetone: After defatting, the polar phenolics were extracted using an aqueous acetone solution (30/70 v/v H2O/acetone), followed by sonication and centrifugation. The supernatant was then evaporated, freeze-dried, and re-dissolved in a dilute H2O/MeOH solution for analysis. This selective extraction method was designed to concentrate the target compounds and minimize interference.
  1. Comprehensive Two-Dimensional Liquid Chromatography (LC × LC): The Ultimate Separator
    Traditional one-dimensional (1D) liquid chromatography often struggles with complex plant extracts, as many compounds co-elute (come out of the column at the same time), making individual identification nearly impossible. 2D-LC overcomes this limitation by employing two different separation mechanisms in sequence:
  • First Dimension (¹D) – Hydrophilic Interaction Liquid Chromatography (HILIC):
    • Mechanism: HILIC separates compounds based on their polarity. Polar compounds interact strongly with the stationary phase (Acquity BEH Amide column, 150 × 1.0 mm, 1.7 µm) and are retained longer, while less polar compounds elute faster.
    • Purpose: This step effectively “spreads out” the highly polar phenolic compounds, providing an initial broad separation based on a property distinct from the second dimension.
    • Dilution and Modulation: The effluent from the ¹D column was diluted with a weak reversed-phase solvent and then introduced into an interface with two 80 µL loops, acting as a modulator. This process collects small fractions from the ¹D separation and rapidly injects them onto the ²D column, preventing peak distortion.
  • Second Dimension (²D) – Reversed-Phase Liquid Chromatography (RP-LC):
    • Mechanism: RP-LC separates compounds based on their hydrophobicity. Less polar compounds are retained longer on the stationary phase (Zorbax Eclipse Plus C18 column, 50 × 3.0 mm, 1.8 µm), while more polar compounds elute faster.
    • Purpose: By applying a different separation mechanism, RP-LC can resolve compounds that may have co-eluted in the HILIC dimension.
    • Fast Gradient: The ²D separation uses a very fast gradient (0.45 min) and a high flow rate (3 mL/min) to ensure rapid analysis of each ¹D fraction, maintaining high resolution.
  • Orthogonality and Peak Capacity: The combination of HILIC and RP-LC is highly “orthogonal” because these two modes separate compounds based on fundamentally different chemical properties. This means compounds that co-elute in one dimension are highly likely to be separated in the other, leading to vastly improved resolution. The Stellenbosch method achieved an “excellent separation performance” with a “practical peak capacity above 3000” and an average orthogonality of 75%. This level of separation is exponentially greater than what can be achieved with 1D methods, allowing for the detection of many more individual compounds.
  • Method Optimization: The team used an in-house developed predictive optimization algorithm (in Matlab R2019b) [31-33]. This software systematically explored a wide range of experimental conditions to find the optimal settings for analysis time, peak capacity, and resolution, further enhancing the method’s effectiveness.
  1. High-Resolution Mass Spectrometry (HR-MS) – Quadrupole Time-of-Flight (Q-TOF): Identifying the Unknowns
    As compounds exit the ²D column, they are immediately directed to a Q-TOF mass spectrometer. This instrument is essential for identifying the separated compounds:
  • Accurate Mass Measurement: Q-TOF provides highly accurate mass measurements of both precursor (intact) ions and fragment ions. This precision allows researchers to determine the exact molecular formula of an unknown compound, which is a crucial first step in identification.
  • MSE Fragmentation: The instrument was operated in MSE mode, which collects both low (4 eV) and high (10-30 eV ramped) collision energy data simultaneously. Low-energy data shows the intact molecular ions, while high-energy data provides characteristic fragmentation patterns. These “fingerprints” are invaluable for elucidating the structure of compounds, even those never before seen.
  • Tentative Identification: By combining accurate mass data, fragmentation patterns, UV spectral data (from the DAD detector), and relative retention times in both dimensions, researchers could tentatively identify 79 compounds, including the novel flavoalkaloids and phenolic acid sulfates.

This sophisticated analytical pipeline allowed the Stellenbosch team to peer into the complex chemistry of cannabis with unprecedented clarity, leading to discoveries that would have been impossible with less advanced techniques.

rare compounds in cannabis

The Road Ahead: An Exciting Journey of Discovery

The work by Magriet Muller and Professor André de Villiers is not merely an academic exercise; it is a powerful stride into the future of cannabis. It reminds us that even in plants we think we know well, nature often holds profound secrets waiting to be uncovered. As research continues to peel back the layers of Cannabis sativa’s intricate chemistry, the potential for new health solutions and economic opportunities grows exponentially.

For South Africa, this research is a beacon of hope and innovation. It champions local scientific excellence and offers a path toward a future where cannabis is understood and utilised for its full, multifaceted potential, contributing significantly to health, wellness, and a sustainable economy. The journey to fully understand cannabis and its immense potential has just become even more fascinating, and we eagerly await the next chapter of discoveries that will undoubtedly emerge from our vibrant scientific community.

You can read the full published paper here: “Comprehensive two-dimensional liquid chromatographic analysis of Cannabis phenolics and first evidence of flavoalkaloids in Cannabis” by Magriet Muller and André de Villiers, 2 August 2025, Journal of Chromatography A.
DOI: 10.1016/j.chroma.2025.466023

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Unlocking Growth: Deep Dive into Plant Growth Regulators (PGRs)

plant growth regulators

In the pursuit of optimal yields and pristine quality, cannabis cultivators are constantly seeking every advantage. While dialling in light, nutrients, and environmental control are standard practice, the world of Plant Growth Regulators (PGRs) introduces a powerful, yet often misunderstood, layer of influence. For many growers, the term “PGR” can spark confusion or even controversy. Some might unknowingly be using them through common supplements, while others might dismiss them without a full understanding of their intricate effects.

This week, we’re taking a candid look at PGRs. This isn’t about judgment, but about illuminating the science behind these potent compounds so you, as a grower, can make informed decisions in your cultivation space. We’ll explore what PGRs are, how they interact with your cannabis plants, and the critical considerations for their responsible and effective use.

What Are Plant Growth Regulators (PGRs)?

At their core, PGRs are simply phytohormones – naturally occurring chemical messengers that regulate virtually every aspect of plant growth, development, and physiological response. Plants produce these hormones internally in minute quantities, but even these tiny amounts orchestrate monumental changes, from seed germination and root formation to flowering and senescence.

The “PGRs” we discuss in cultivation refer to these phytohormones, whether they are:

  • Naturally derived: Extracted from organic sources that are rich in these compounds.
  • Synthetic: Chemically synthesised compounds designed to mimic the effects of natural phytohormones.

Understanding that these substances are, fundamentally, plant hormones is key. When applied externally, they can dramatically influence the plant’s natural processes.

plant growth regulators

The Master Conductors: Auxins, Cytokinins, and Gibberellins

Three classes of phytohormones stand out as the primary conductors of plant growth, each with distinct roles and complex interactions:

1. Auxins: The Architects of Direction

Auxins, such as Indole-3-acetic acid (IAA) and 1-naphthaleneacetic acid (NAA), are largely responsible for directing plant growth from the shoot apex downwards. Their key roles include:

  • Apical Dominance: Auxins produced at the shoot tip inhibit the growth of lateral (side) branches, promoting a single dominant main stem. This is why “topping” or removing the apical meristem is a common technique to break apical dominance and encourage bushier growth.
  • Root Development: Auxins are crucial for initiating and stimulating the growth of adventitious and lateral roots, making them vital in cloning and tissue culture processes.
  • Cell Elongation: They promote the lengthening of plant cells, contributing to overall plant height.

These vital phytohormones are not only responsible for directing shoot growth and root initiation but are also deeply involved in the very earliest stages of plant architecture. Their precise transport mechanisms, involving specialised protein pathways in both the phloem and through cell-to-cell polar transport, allow them to create gradients crucial for everything from defining the apical-basal axis in early embryogenesis to guiding the differentiation of vascular tissues throughout the plant. This complex internal movement ensures that growth is organised and resources are distributed effectively, shaping the fundamental structure your plant will build upon.

2. Cytokinins: The Stimulators of Division and Lateral Growth

Cytokinins (CKs), such as 6-benzylaminopurine (BAP) and kinetin, are primarily synthesized in root tips and young, actively dividing tissues. Their main functions are:

  • Cell Division: Cytokinins vigorously stimulate cell division (cytokinesis), a fundamental process for tissue formation and plant growth.
  • Lateral Bud Break: They promote the growth of lateral buds, effectively counteracting the apical dominance maintained by auxins, leading to bushier plants.
  • Delaying Senescence: Cytokinins help to maintain chlorophyll content and delay leaf ageing, keeping leaves greener for longer periods and enhancing photosynthetic activity.

While known for driving cell division and promoting lateral branching, cytokinins also play a direct role in maintaining the photosynthetic machinery of the plant. They do this by actively slowing down the enzymatic degradation of chlorophyll, keeping leaves greener for extended periods. Furthermore, cytokinins stimulate the opening of stomata, particularly in mature and ageing leaves, which directly influences the plant’s uptake of carbon dioxide for photosynthesis. This dual action on chlorophyll and stomatal function underscores their importance in sustaining the plant’s energy production efficiency.

3. Gibberellins (GAs): The Accelerators of Elongation and Flowering

Gibberellins (GAs), including Gibberellic Acid (GA3), are potent growth promoters known for:

  • Stem Elongation (Bolting): GAs are powerful stimulators of internode elongation, leading to increased plant height. This process is often called “bolting” and is crucial in many plant life cycles.
  • Germination and Flowering: GAs break seed dormancy and stimulate germination. They also play a role in initiating flowering, particularly in long-day plants, by mediating photoperiod signals.

Beyond their well-known role in stem elongation and breaking seed dormancy, Gibberellins significantly influence various reproductive traits. In cannabis, GAs have been shown to influence sex determination, with certain applications potentially increasing the proportion of male flowers. Their impact also extends to seed productivity; while often associated with increased growth, studies have revealed that Gibberellin applications can sometimes lead to lower seed mass in treated plants, highlighting a complex interaction with reproductive development that can vary by genotype and application method.

plant growth regulators

The Orchestration of Growth: Interplay and Balance

These phytohormones do not act in isolation. They form a complex regulatory network where their effects are interdependent:

  • Auxin-Cytokinin Balance: The ratio of auxins to cytokinins is critical. A high auxin-to-cytokinin ratio promotes root growth, while a low ratio favours shoot development and lateral branching. They often work antagonistically to control processes like apical dominance, but can also be synergistic, for example, in promoting vascular tissue formation.
  • GA Interactions: GAs often interact with both auxins and cytokinins to regulate overall plant architecture and reproductive development. As Alter et al. (2024) found, Gibberellins reduce auxin levels in the cannabis shoot apex under short-day conditions, contributing to a condensed inflorescence structure. Conversely,an  external GA application can impede proper inflorescence development by promoting stem elongation.

This intricate balance is why the intentional application of PGRs must be approached with precision and a deep understanding of their roles.

PGRs and Cannabis: Influencing Morphology, Yield, and Cannabinoids

For cannabis growers, the application of exogenous PGRs aims to modify plant architecture, optimise yields, and even influence cannabinoid content. Recent research offers crucial insights:

  • Modifying Plant Architecture: Studies have shown that applying auxins (like NAA) and cytokinins (like BAP), either alone or in combination, can significantly reduce overall plant height, internode length, and axillary branch length in cannabis. For instance, Burgel et al. (2020) and Mishchenko et al. (2022) observed that NAA and BAP applications resulted in shorter, more compact cannabis plants with reduced branching, achieving a compact growth habitus desired for indoor cultivation with limited space. This ability to make plants bushier and more manageable without reducing overall flower yield per unit area is a key benefit for many growers.
  • Impact on Cannabinoid Content: The influence of PGRs on cannabinoid content is more complex and can be genotype-specific. Mishchenko et al. (2022) found that while ascorbic acid, auxins, and Gibberellins significantly reduced cannabinoid content in hemp, nicotinic acid and cytokinins (BAP) actually increased it. Notably, BAP treatments significantly boosted cannabidiol (CBD) levels. Conversely, Alter et al. (2024) observed that exogenous GA and auxin negatively affected total cannabinoid content in their cannabis cultivar. These conflicting results underscore that the precise effect of cannabinoids depends heavily on the specific PGR, its concentration, the plant’s genotype, and the timing of application.
  • Yield Parameters: While some PGRs can reduce plant height, they often do not negatively impact the total inflorescence yield per plant or per unit area. In some cases, as Burgel et al. (2020) reported, even with reduced plant height and branching, the inflorescence dry weight yield remained similar to untreated control plants.
plant growth regulators

The Crucial Distinction: Natural vs. Synthetic PGRs

The term “PGR” often conjures images of harsh chemicals, leading some growers to believe they are avoiding PGRs while unknowingly using them. This distinction is vital:

  • Naturally Derived PGRs: Many popular organic supplements, such as seaweed or kelp extracts, are rich sources of natural auxins, cytokinins, and Gibberellins. These products are indeed PGRs, as they introduce plant hormones that influence growth. The key difference is often their concentration and the presence of other beneficial compounds that can buffer their effects.
  • Synthetic PGRs: These are laboratory-synthesised compounds designed for specific, often more potent, effects. Examples include paclobutrazol and daminozide. While some are used in ornamental horticulture to control plant size, many are not approved or safe for use on consumable crops due to potential health concerns regarding residues.

The misconception that “natural” means “not a PGR” is widespread. All substances that introduce phytohormones, whether extracted or synthesised, are indeed PGRs. The concern lies not in the label but in the compound itself, its concentration, and its intended application. Products containing synthetic PGRs, particularly those not approved for consumable crops, pose risks due to potential toxic residues that can affect human health (e.g., impact liver or kidney function) or alter the final product’s quality and taste. Legal, regulated cannabis markets typically impose strict testing for such residues.

A Grower’s Responsibility: Precision, Knowledge, and Intent

Using PGRs in cannabis cultivation demands meticulous attention and a commitment to understanding their profound effects. Here are key takeaways for responsible cultivation:

  • Knowledge is Power: Understand the specific roles of auxins, cytokinins, and Gibberellins, and how they interact. Research the precise effects of any PGR product you intend to use.
  • Genotype Specificity: Recognise that different cannabis strains (chemovars) will react uniquely to PGRs. What works for one strain may not work for another. Genotype-specific responses are a recurring theme in cannabis research.
  • Precise Application: Dosage, timing, and method of application (foliar spray vs. root drench) are critical. Even small deviations can lead to unintended or adverse effects on plant morphology, yield, and cannabinoid content.
  • Monitor and Document: Keep detailed records of your applications and plant responses. This allows you to observe subtle changes and refine your strategy over time, adapting to your specific cultivar and environment.
  • Holistic Management: PGRs are just one tool. They work best within a comprehensive strategy that includes optimised lighting, precise nutrient management (remembering that NPK balance can influence endogenous hormone pathways), and diligent environmental control.
  • Prioritise Safety and Quality: Always choose products from reputable suppliers who provide lab testing. Be wary of unverified claims. The goal is to enhance plant growth without compromising the safety or quality of your final product.

PGRs are powerful tools that, when used with respect and deep understanding, can revolutionise your cultivation practices. By embracing the science behind these potent compounds, you can unlock new levels of plant control, optimising your cannabis grow to meet your specific goals while ensuring a healthy, high-quality harvest.

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CBG, The Original Cannabinoid

CBG, The Original Cannabinoid

We can all agree that most of the spotlight has traditionally shone on two major compounds: the psychoactive THC and the calming CBD. But as science and consumer curiosity evolve, we are beginning to appreciate the vast and complex family of over 100 cannabinoids found in the plant. Among these, one compound stands out for its foundational role and unique potential: Cannabigerol (CBG).

Often called the “stem cell of all cannabinoids,” CBG is a non-intoxicating compound that acts as the chemical precursor from which many other major cannabinoids are synthesised within the cannabis plant. While it’s typically found in smaller quantities in mature plants, its potential therapeutic benefits and unique interactions with our bodies are generating significant excitement.

This guide will provide a deep dive into what CBG is, how it works, its potential benefits as highlighted by recent research, and its unique place within the broader cannabinoid family.

What is CBG, and Why is it Called “The Stem Cell”?

CBG’s story begins with its acidic form, Cannabigerolic Acid (CBGA). Within the growing cannabis plant, CBGA is the first major cannabinoid acid to form. It serves as a crucial building block. As the plant matures, natural enzymes synthesise other cannabinoid acids from CBGA, primarily:

  • Tetrahydrocannabinolic Acid (THCA), which becomes THC when heated.
  • Cannabidiolic Acid (CBDA), which becomes CBD when heated.
  • Cannabichromenic Acid (CBCA), which becomes CBC when heated.

Because CBGA is the starting point for these major compounds, it is often referred to as the “mother” or “stem cell” cannabinoid. Any CBGA that is not converted into these other forms will, upon heating (decarboxylation), become CBG. This is why most finished cannabis flower contains high levels of THC or CBD but only trace amounts of CBG—most of it has already been transformed.

However, breeders are now developing CBG-dominant strains, and extractors are isolating it, allowing us to explore the unique properties of this foundational molecule on its own. And please, let me say this again. I support the isolation of compounds to study their effects. However, I don’t believe in medication through isolation. Full-spectrum medication is what I believe in.

How Does CBG Interact with Our Bodies? A Unique Mechanism

Like other cannabinoids, CBG interacts with our body’s Endocannabinoid System (ECS), the master regulatory network responsible for maintaining internal balance (homeostasis). But CBG’s method of interaction is distinctly different from that of THC or CBD.

  • THC primarily acts by directly binding to and activating the CB1 receptor, producing strong psychoactive effects.
  • CBD has a very low affinity for CB1 and CB2 receptors. As we’ve discussed, one of its key actions is inhibiting the FAAH enzyme, which increases levels of our body’s own anandamide.

CBG, on the other hand, exhibits a broader and more complex range of interactions:

  • It acts as a partial agonist for both CB1 and CB2 receptors, meaning it can bind to them but doesn’t produce the strong intoxicating effect of THC.
  • Crucially, research has shown CBG to be a potent alpha-2-adrenergic receptor (α2-AR) agonist. These receptors are involved in regulating sympathetic nerve activity, which controls processes like heart rate and blood pressure. This unique action is not seen with other major cannabinoids and is a key area of research for potential cardiovascular applications.
  • It also interacts with serotonin receptors (as a 5-HT1A antagonist) and various TRP channels, which are involved in mediating pain, inflammation, and temperature sensation.

This multi-target mechanism means CBG has a unique and versatile potential to influence our physiology in ways that differ from its more famous counterparts. To learn more about CBG, click here to download a Study Review.

CBG, The Original Cannabinoid

Exploring the Potential Benefits of CBG

While human clinical research on CBG is still in its early stages, preclinical studies (in vitro and in animal models) and initial human trials have highlighted several promising areas where CBG may offer therapeutic benefits.

1. Anti-Inflammatory and Antioxidant Properties

Much like CBD, CBG has demonstrated significant anti-inflammatory and antioxidant effects in laboratory settings. It has been shown to reduce the production of inflammatory cytokines and inhibit oxidative stress by neutralising reactive oxygen species (ROS). This action is central to its potential in managing a variety of inflammatory conditions, from skin disorders to inflammatory bowel disease.

2. Potential in Pain Management and Stress Relief

CBG is gaining attention for its analgesic (pain-relieving) properties. A recent double-blind, placebo-controlled clinical trial with healthy adults found that a 20 mg dose of CBG significantly reduced subjective feelings of anxiety and stress compared to a placebo. This human trial corroborates earlier survey data where individuals reported using CBG successfully for managing anxiety.

Furthermore, a pioneering study on horses with chronic osteoarthritis provided compelling evidence. Horses given an oil containing both CBG and CBD showed a significant reduction in pain scores and a decrease in inflammatory markers in their blood, without any adverse side effects. This not only supports CBG’s potential for pain modulation but also highlights its good safety profile in veterinary applications.

3. Neuroprotective Effects

Preclinical research suggests that CBG has neuroprotective qualities, meaning it may help protect nerve cells from damage. This has led to its investigation for neurodegenerative conditions, though this research is still very early.

4. Cardiovascular Health

Because of its unique action as an α2-AR agonist, CBG is being explored for its potential to lower blood pressure. While initial studies in mice have shown hypotensive effects, more research is needed to understand how chronic administration of CBG would affect cardiovascular parameters in humans, especially those with hypertension.

CBG, The Original Cannabinoid

The Bigger Picture: CBG, CBDA, and the Power of the “Entourage”

The recent study on horses is particularly insightful because it didn’t just test one compound. It compared two different formulations: one with CBDA (the acidic precursor to CBD) and another with a combination of CBG and CBD. Both treatments were effective in reducing pain, but the study design underscores a key concept in cannabis science: the “entourage effect.“

Just as terpenes can modulate the effects of cannabinoids, different cannabinoids can work together synergistically. Combining CBG and CBD may offer a broader spectrum of action than either compound alone. For example, CBG’s unique receptor interactions combined with CBD’s well-documented anti-inflammatory properties could create a more comprehensive therapeutic effect.

This highlights the value of full-spectrum or broad-spectrum products, which retain a range of cannabinoids and terpenes, versus isolates, which contain only a single compound.

CBG, The Original Cannabinoid

Your Guide to Exploring CBG

As CBG becomes more available in tinctures, edibles, and even flower, here’s how you can approach it mindfully:

  • Start with a Reputable Source: Ensure any CBG product you purchase is from a well-respected Rasta, Budtender shop or healer. Have a conversation with the person about the CBG, and ask about lab testing. Ask about sourcing and extraction. When you get an honest answer, you will be able to make an informed decision.
  • Understand the Dose: As the clinical trial showed, even a relatively low dose of 20 mg can produce noticeable effects on stress and anxiety. Always start low and go slow, especially if you are new to CBG, to gauge your individual response. Trust me on this. My first time smoking CBG flower, I was blown away by how much of a clear mental state it gave me, and it was a little uneasy. Ease yourself into it. 
  • Consider the Goal: Are you looking for stress relief, pain modulation, or general wellness? Your intent can guide your choice between a CBG-isolate product or a broad-spectrum product containing CBG alongside other cannabinoids like CBD.
  • Manage Expectations: While the research is exciting, it’s still emerging. CBG is not a cure-all, and its effects can be subtle and vary from person to person.

CBG, the “stem cell cannabinoid,” is finally stepping into the spotlight, revealing itself as a compound with a unique and promising profile. As science continues to unravel the complex chemistry of cannabis, we are reminded that there is so much more to this plant than just THC and CBD. Exploring compounds like CBG opens a new chapter in our understanding of how cannabis can contribute to health and well-being.

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Understanding and Combating Bud Rot in Your Cannabis Grow

Bud Rot

For any cannabis cultivator, few discoveries are as disheartening as the appearance of bud rot within a flourishing canopy. What begins as a subtle discolouration can swiftly spread, transforming dense, resinous flowers into a soft, grey, unusable mass. This destructive disease, caused by the pervasive fungal pathogen Botrytis cinerea, represents one of the most significant threats to cannabis crops globally, capable of inflicting substantial losses both before and after harvest.

Understanding this notorious fungus – its intricate life cycle, the environmental conditions it thrives in, and its mechanisms of infection – is the most critical step in safeguarding your valuable crop. This guide, drawing on extensive scientific research, will provide an in-depth look at Botrytis cinerea to help you proactively identify, manage, and ultimately prevent this silent threat in your garden.

What is Botrytis cinerea?

Botrytis cinerea, commonly known as grey mould or bud rot, is a highly successful and ubiquitous necrotrophic fungus. This means it obtains its nutrients by actively killing host plant cells and feeding on the dead tissue. It boasts an incredibly vast host range, capable of infecting over 1,000 plant species, including economically significant agricultural crops like strawberries, grapes, and tomatoes. In cannabis, it is the primary culprit behind bud rot, specifically targeting the dense, moisture-retaining inflorescences.

The fungus is a master of survival, utilising various forms and strategies to persist and spread, making it a persistent challenge in both indoor and outdoor cultivation environments.

The Life Cycle of Bud Rot: From Microscopic Spore to Devastating Decay

To effectively combat Botrytis, a clear understanding of its operational blueprint is essential. The diagram below illustrates the typical disease cycle, which, under ideal conditions, can progress from initial infection to full bud rot in as little as 3–4 days.

bud rot

A Step-by-Step Breakdown of the Cycle:

  • Inoculum and Spore Landing: The cycle almost always begins with microscopic, airborne spores called conidia. These conidia are the primary means of transmission and are a constant presence in the environment. They can originate from resilient overwintering structures known as sclerotia (hardened masses of fungal mycelium) in soil or plant debris, or from previously infected plant material. Carried by wind or splashing water, these spores land on susceptible parts of the cannabis plant.
  • Spore Germination and Penetration: For the dormant spore to activate and germinate, it requires specific conditions, primarily sustained moisture and an accessible nutrient source. Once hydrated, the spore produces a germ tube that initiates penetration into the plant tissue. In cannabis, the exposed stigmas (pistils) of young flowers often serve as a prime entry point, providing both moisture and a rich nutrient source. The fungus employs a combination of physical pressure and enzymatic activity (releasing cell-wall-degrading enzymes) to breach the plant’s outer defences.
  • Colonisation and Early Infection: Once inside the plant, Botrytis begins to colonise the host tissue. It can initially enter a biotrophic phase, where it lives within the plant without immediately killing cells, often suppressing the plant’s initial defence responses. This can lead to latent infections, where the fungus is present but no visible symptoms are apparent, complicating early detection.
  • Necrosis and Bud Rot Development: Triggered by factors such as tissue maturity (e.g., ripening flowers), senescence, or environmental stress, the fungus switches to its aggressive necrotrophic phase. It begins to secrete phytotoxins (like botryoidal and botcinic acid) and enzymes that actively kill plant cells. This is the stage where the familiar symptoms of bud rot emerge: water-soaked spots, rapid browning and softening of tissues, and the eventual decay and collapse of the entire flower structure.
  • Reproduction and Spore Dissemination: As the fungus consumes the dead tissue, it rapidly produces new conidiophores – structures that bear masses of new conidia. These grey, fuzzy structures emerge from the decaying bud, releasing a fresh wave of spores into the air, ready to infect adjacent buds or other plants. This secondary spread is rapid and devastating.
  • Overwintering: If infected plant material is left behind (e.g., in soil or on surfaces), the fungus can form resilient sclerotia. These dormant structures allow Botrytis to survive harsh conditions, awaiting favourable temperatures and moisture to initiate new infections in subsequent growing seasons.

Environmental and Plant Factors Driving Bud Rot

Botrytis cinerea is not an all-powerful entity; its success hinges on the presence of specific environmental conditions. For growers, manipulating these factors is the most potent line of defence against infection.

  • Humidity is the Prime Culprit: High relative humidity (RH) is the single most critical factor for Botrytis spore germination and mycelial growth. Levels above 85–90% RH are highly conducive to its development. Conversely, maintaining RH below 65% in your grow environment can effectively inhibit the fungus. As discussed in our previous blog on burping, controlling humidity is paramount for preventing mould and maintaining bud quality.
  • Temperature’s Influence: Botrytis is generally a cool-season disease. Its optimal temperature range for growth and sporulation is typically between 15–22°C (59–72°F). While it can survive cooler conditions, its growth is significantly reduced at temperatures above 30°C (86°F). This highlights a balancing act for growers, especially in indoor environments, to find the sweet spot that favors plant growth but inhibits pathogen development.
  • Moisture and Leaf Wetness: Any form of free moisture on the plant surface – whether from condensation (due to sharp temperature drops), guttation (exudation from leaves overnight), or improper watering – provides the essential hydration for spores to germinate and infect. Prolonged periods of leaf wetness are a major risk factor.
  • Air Circulation and VPD: Poor air movement and stagnant conditions create humid microclimates around plants, especially within dense canopies. Maintaining adequate Vapour Pressure Deficit (VPD) is crucial for healthy plant transpiration and for keeping plant surfaces dry. Circulating fans and proper ventilation are essential tools to prevent these humid pockets from forming.
  • Plant Architecture and Density: The physical structure of your cannabis plants directly impacts susceptibility. Strains that produce very large, dense, and compact inflorescences with many overlapping leaves are inherently more susceptible to bud rot. This dense architecture traps moisture and hinders airflow, creating ideal microclimates for the fungus.

The Nuance of Plant Chemistry: Cannabinoids, Terpenes, and Defence

While cannabis plants produce a complex array of secondary metabolites, including cannabinoids and terpenes, their direct role in defending against Botrytis cinerea is a nuanced and ongoing area of research.

Antifungal Properties of Terpenes/Essential Oils: Studies on essential oils derived from other plants (like tea tree oil, oregano, cinnamon, and thyme) have demonstrated strong antifungal activity against Botrytis cinerea in laboratory settings. These terpenes can disrupt fungal cell walls and membranes. While cannabis itself contains many of these same terpenes, direct evidence for their efficacy against Botrytis on the cannabis plant is still being explored. Some research indicates that specific terpene profiles or their emissions from maturing inflorescences might even influence Botrytis development on cannabis, though results are not always consistent.

Cannabinoids and Resistance: Similarly, the direct fungicidal effect of cannabinoids like THC and CBD against Botrytis on the living plant remains a complex area of study. While cannabis plants do possess innate defence responses at the biochemical and molecular levels (involving hormonal pathways like Jasmonic Acid and Ethylene, and Salicylic Acid), no known cannabis resistance genes to Botrytis have been conclusively identified yet. This means relying solely on the plant’s inherent chemical defenses for bud rot prevention is not a primary, reliable strategy.

This understanding underscores that while cannabis is a biorefinery of fascinating compounds, a holistic IPM strategy, rather than just chemical composition, is the most robust defence against Botrytis.

bud rot

Integrated Management: A Proactive, Multi-Layered Defence

Given that Botrytis cinerea is notoriously difficult to eradicate once established, prevention is always the most effective strategy. A comprehensive and proactive Integrated Pest Management (IPM) program is essential. This combines various approaches to create an environment where Botrytis simply cannot thrive.

Cultural Practices: These focus on optimising the grow environment and plant health:

  • Strategic Strain Selection: Prioritise genetics that naturally exhibit a more open, less compact bud structure, which is less conducive to moisture retention.
  • Meticulous Sanitation: Regularly remove all dead or dying plant material, fallen leaves, and debris from your grow space. This eliminates potential food sources and inoculum for the fungus.
  • Targeted Defoliation and Pruning: Thoughtful defoliation, especially in the lower and inner canopy, improves airflow, reduces humidity pockets, and allows better light penetration. Pruning should be clean to avoid creating open wounds.
  • Optimal Plant Density: Avoid overcrowding plants in your grow space. Proper spacing significantly enhances air circulation and light exposure throughout the canopy.
  • Nutrient Management: While direct links between specific nutrients and Botrytis resistance in cannabis can be conflicting, maintaining overall plant health through balanced nutrition (avoiding extremes, as discussed in our NPK optimisation guide) inherently boosts a plant’s resilience to stress and pathogens.

Environmental Modification: Precise control of atmospheric conditions is paramount:

  • Rigorous Humidity Control: This is your primary defence. Utilise dehumidifiers, proper exhaust, and oscillating fans to consistently keep RH below 65% during flowering, and avoid sharp RH spikes.
  • Optimised Temperature Control: Maintain temperatures within the optimal range for cannabis growth while being mindful of night-time drops that can lead to condensation and conditions favourable for Botrytis.
  • Enhanced Airflow: Ensure continuous, gentle air movement across and within the canopy. This prevents stagnant air pockets and promotes rapid drying of any moisture on plant surfaces.
  • Biological Control Agents (Biofungicides): This approach offers a powerful, reduced-risk alternative or supplement to other strategies:
  • Harnessing Beneficial Microbes: Biofungicides utilise specific strains of beneficial bacteria (like Bacillus and Pseudomonas) and fungi (like Trichoderma) that can naturally suppress Botrytis.
  • Mechanisms of Action: These agents work through various means: by competing with Botrytis for space and nutrients, by producing antifungal compounds that directly inhibit the pathogen, or by triggering the plant’s Induced Systemic Resistance (ISR), essentially “priming” the plant’s own defence mechanisms to better fight off invaders. Several such products are approved for use on cannabis in various regions, offering a clean, effective layer of protection.

Post-Harvest Management: The Final Stand: The battle against bud rot extends beyond harvest.

  • Proper Drying Techniques: Your drying space must have excellent airflow and be maintained at appropriate temperature and humidity levels to dry buds efficiently and thoroughly. This minimises the window for Botrytis to proliferate.
  • Controlled Curing: As emphasised in our burping discussions, curing must be done in a controlled environment with proper burping or humidity control packs (like Boveda) to prevent moisture accumulation, which can reactivate dormant mould spores and lead to costly product recalls.
  • Rigorous Inspection: Inspect every bud during trimming and curing. Any sign of rot, no matter how small, must be immediately removed and discarded to prevent it from spreading to healthy material.
bud rot

Cultivating Resilience and Protecting Your Harvest

The threat of Botrytis cinerea is a constant challenge for cannabis cultivators, capable of undermining months of hard work. However, armed with scientific knowledge and a proactive mindset, it is a challenge that can be overcome. The key lies not in a single solution, but in a holistic and integrated IPM strategy.

By intentionally selecting resistant strains, meticulously controlling your environment, implementing diligent cultural practices, and leveraging the power of biological controls, you transform your grow space into an inhospitable fortress against Botrytis. This multi-layered defence, coupled with unwavering vigilance from propagation to post-harvest, empowers you to protect your plants and ensure your efforts culminate in the clean, healthy, and high-quality buds you aspire to cultivate.

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Anandamide: Unlocking the “Bliss Molecule”

Anandamide: Unlocking the "Bliss Molecule"

In the vast landscape of the human body’s biochemistry, few molecules have as intriguing a name as anandamide. Derived from the Sanskrit word “ananda,” meaning “internal bliss” or “joy,” this compound is a cornerstone of a critical regulatory network known as the Endocannabinoid System (ECS). While the ECS gained fame through its connection to cannabis, understanding anandamide itself is key to unlocking the science behind our body’s sense of balance, well-being, and how it responds to cannabinoids like THC and CBD.

This post will dive into what anandamide is, how it functions within your body, and its intricate relationship with cannabis, from the plant’s psychoactive effects to its therapeutic potential.

What is Anandamide?

Anandamide (AEA) is an endocannabinoid, meaning it’s a cannabinoid-like molecule produced inside your body. Discovered in the early 1990s, it was one of the first endogenous “keys” found that fit the “locks” of the cannabinoid receptors, which had just been identified as the primary targets of THC.

Anandamide was the proof. It was the body’s own, internally produced molecule that perfectly fit into the CB1 receptor lock. This confirmed that the cannabinoid receptors weren’t just for cannabis; they were part of a vast, pre-existing communication network essential for our health – The Endocannabinoid System.

So, when we say:

“Anandamide (AEA) was one of the first endogenous ‘keys’ found that fit the ‘locks’ of the cannabinoid receptors, which had just been identified as the primary targets of THC.”

We are saying this:

Scientists first found the “lock” (the CB1 receptor) by seeing where THC from cannabis was binding in the brain.

They correctly assumed our bodies must have a natural reason for these locks.

They then discovered Anandamide – the first internally-produced “key” that our body makes to regulate itself by unlocking these same receptors.

Anandamide: Unlocking the "Bliss Molecule"
Anandamide: Unlocking the "Bliss Molecule"

Unlike traditional neurotransmitters that are stored in vesicles and released when needed, anandamide is synthesised on demand. When your body senses a need to restore balance, whether in response to stress, pain, or inflammation, your cells produce and release anandamide. It has a short half-life and is quickly broken down by an enzyme called Fatty Acid Amide Hydrolase (FAAH). This rapid synthesis and degradation allow for precise, localised control over various physiological processes.

The Role of Anandamide and the Endocannabinoid System

Anandamide and the broader ECS act as a master regulatory system, helping to maintain homeostasis (internal balance). Research has shown it plays a vital role in modulating a wide range of functions:

  • Mood and Anxiety: Studies have consistently linked higher anandamide levels with reduced anxiety. Research in both animal models and humans has shown that elevating anandamide levels, for instance through exercise or by inhibiting the FAAH enzyme, produces anxiolytic (anxiety-reducing) effects. In fact, some individuals with naturally higher anandamide levels (due to a genetic variation in the FAAH enzyme) self-report lower anxiety.
  • Pain and Inflammation: The ECS is deeply involved in modulating pain signals and inflammatory responses. Anandamide can help regulate these processes, which is why cannabinoid-based therapies are being explored for pain management and inflammatory conditions.
  • Reward and Motivation: The ECS, including anandamide, plays a modulatory role in the brain’s reward circuitry. It can influence how we experience pleasure and motivation, a factor that is central to research on addiction and substance use disorders.
  • Sleep: As some of the provided research highlights, anandamide is also implicated in sleep regulation. Studies have shown that administration of anandamide can induce sleep and increase slow-wave (deep) sleep, possibly by influencing adenosine levels, another key sleep-promoting molecule.
  • Appetite and Metabolism: Anandamide is known to stimulate appetite, a well-known effect also associated with THC.
Anandamide: Unlocking the "Bliss Molecule"

The Connection to Cannabis: How THC and CBD Interact with Anandamide

The cannabis plant produces phytocannabinoids (plant-based cannabinoids) that interact with our ECS, often by mimicking or influencing anandamide.

  • THC and Anandamide: THC, the main psychoactive component of cannabis, is a partial agonist of the CB1 receptor – the same receptor that anandamide activates. Essentially, THC fits into the same “lock” as anandamide, but it does so more powerfully and for a longer duration, as it’s not broken down as quickly. This strong activation of CB1 receptors, particularly in the brain, is what produces the euphoric “high” and other effects associated with cannabis, such as increased appetite and altered perception. Chronic, heavy cannabis use can lead to the brain downregulating its CB1 receptors to compensate for this constant stimulation. In turn, this can lead to lower anandamide levels in some individuals, a finding that is particularly relevant in studies of cannabis use disorder.
  • CBD and Anandamide: Unlike THC, CBD does not bind strongly to CB1 receptors and is non-intoxicating. Instead, one of its primary mechanisms of action is to inhibit the FAAH enzyme, the very enzyme that breaks down anandamide. By slowing down FAAH’s activity, CBD can lead to an increase in your body’s own anandamide levels. This is a crucial distinction: instead of directly activating the receptors like THC, CBD helps boost your natural “bliss molecule.”

This FAAH-inhibiting action is a key hypothesis behind many of CBD’s potential therapeutic benefits. For example, research has explored CBD’s role in treating psychiatric disorders. A recent clinical trial investigated this very mechanism in individuals with cannabis use disorder, looking at how CBD administration affects plasma anandamide levels. The study found that an 800 mg dose of CBD appeared to protect against reductions in anandamide levels that were observed in the placebo group during a cannabis cessation attempt. By potentially increasing anandamide signalling, CBD may help alleviate symptoms of anxiety, psychosis, and withdrawal, offering a promising avenue for treatment.

Anandamide: Unlocking the "Bliss Molecule"

The Takeaway: A Molecule of Balance

Anandamide is more than just our body’s “bliss molecule”; it is a fundamental regulator of our physiological and psychological well-being. It represents the delicate balance our system constantly strives to maintain. The cannabis plant, through compounds like THC and CBD, offers us a way to directly interact with this system. THC acts as a powerful external key, while CBD works more subtly, by helping our own natural key, anandamide, stay in the lock a little longer.

Understanding the role of anandamide deepens our appreciation for both the complexity of our own biology and the profound ways in which cannabis can influence it. Whether you’re a medical user seeking relief or a recreational consumer exploring different experiences, recognising the interplay between anandamide, THC, and CBD can empower you to make more informed and mindful choices on your cannabis journey.

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Is Your Cannabis Consumption Healthy?

cannabis Consumption

In the vibrant, evolving world of cannabis, we often celebrate its incredible versatility – from diverse strains delivering unique experiences to a myriad of consumption methods. Yet, amidst this growth and excitement, there’s a simple, sometimes uncomfortable question that deserves our thoughtful attention: Is your cannabis consumption healthy?

For some, this question might feel intrusive or even offensive. Others might be too shy to answer, or perhaps hold an understandable pride in their self-control. Within our community, a common sentiment is that because cannabis, unlike many substances, cannot be fatally overdosed on, it’s inherently harmless, even in excess. But is that truly the case? Just because you can’t die from cannabis doesn’t necessarily mean limitless consumption is always beneficial for your well-being.

This isn’t a judgment, but an invitation for a candid conversation, coming from a place of genuine care and honesty towards our shared community. Cannabis is indeed an amazing plant, and there’s nothing inherently wrong with enjoying it as much as you desire. However, understanding the when and where of your consumption, and how it aligns with your overall health and goals, is profoundly important.

cannabis consumption

Beyond the “No Overdose” Myth: Defining Healthy Consumption

The fact that cannabis lacks the acute toxicity of many pharmaceuticals or illicit drugs is a significant advantage, often cited as a cornerstone of its safety profile. However, conflating “non-lethal” with “always healthy in any quantity” can create a false sense of security. Healthy consumption isn’t just about avoiding immediate physical harm; it’s about fostering holistic well-being – physical, mental, and social.

To approach this question with nuance, we need to consider several factors that influence how cannabis impacts us:

The Power of Potency: Modern Cannabis is Stronger

Gone are the days of predominantly low-THC flower. Today’s market offers incredibly potent products:

  • High-THC Flower: Strains can now boast THC levels of 20% or even higher, a significant jump from historical averages.
  • Concentrates: Products like BHO, rosin, and diamonds can concentrate THC levels to 70-95% or more. As we’ve explored previously, these extracts are a direct result of advanced processing methods designed to isolate and concentrate cannabinoids and terpenes.

While these high potencies offer profound effects for medical users or experienced recreational consumers, they also demand increased mindfulness. What might have been a moderate dose a decade ago could be a very strong dose today. Consuming such high concentrations frequently without considering individual tolerance can lead to:

  • Increased Psychoactive Effects: Potent THC can intensify experiences, potentially leading to anxiety or paranoia in sensitive individuals, as discussed in our “Choosing the Right Strain” guide.
  • Faster Tolerance Buildup: Regular consumption of high-potency products can lead to a quicker development of tolerance, meaning you need more to achieve the same effects. This can inadvertently increase consumption frequency and quantity.
cannabis consumption

The Method Matters: How You Consume

While we’ve discussed the nuances of different extract types (solvent vs. solventless, the benefits of purity), the method of consumption also plays a role in your health profile:

  • Inhalation (Smoking/Vaping): While smoking cannabis doesn’t carry the same carcinogen risk as tobacco, any form of combustion can introduce respiratory irritants. Vaporizing, on the other hand, is generally considered a cleaner inhalation method as it heats cannabis to produce vapor without burning plant material.
  • Edibles & Topicals: These methods bypass the respiratory system entirely. Edibles, which rely on the body’s digestive system, offer a different onset and duration, while topicals work locally with the skin’s endocannabinoid system without systemic psychoactivity. Choosing a method aligned with your health preferences is part of mindful consumption.

Your Unique Biology: It’s All About “You”

As we emphasized in “Choosing the Right Strain for Your Unique Experience,” the effects of cannabis are profoundly personal. Your individual endocannabinoid system, genetics, metabolism, and even your current psychological state (your “set”) influence how you react. This means:

  • Individual Responses Vary: What’s relaxing for one person might be energizing for another. What helps one person sleep might keep another awake. Healthy consumption requires self-awareness about your unique responses.
  • Tolerance and Dependence: While not all users develop dependence, consistent, high-frequency use can lead to physical and psychological dependence. Understanding this potential and regularly assessing your relationship with cannabis is crucial for maintaining control.
cannabis consumption

Cultivating a Healthy Relationship with Cannabis

This conversation isn’t about shaming or restricting personal choice. It’s about empowering our community with knowledge and encouraging a deeper, more mindful relationship with this remarkable plant. Healthy cannabis consumption is an active, ongoing process of self-awareness and informed decision-making.

Here are a few steps to cultivate a healthier relationship with your cannabis:

  1. Consume with Intent: Before each session, ask yourself: Why am I doing this? What experience am I seeking?
  2. Start Low, Go Slow: Especially with new, high-potency products. This allows you to gauge your individual response and find your optimal dose.
  3. Listen to Your Body and Mind: Pay attention not just to the immediate “high,” but to how cannabis impacts your mood, energy levels, sleep, and overall daily functioning in the hours and days after consumption.
  4. Regular Self-Assessment: Periodically reflect on your consumption habits. Is it enhancing your life? Is it becoming a routine that feels less like a choice and more like a necessity?
  5. Diversify Your Experience: Explore different strains with varied cannabinoid and terpene profiles, and experiment with various consumption methods. This can help prevent tolerance buildup and allow you to find what genuinely supports your well-being.
  6. Seek Professional Guidance: If you have concerns about your cannabis consumption, or are using cannabis for specific medicinal purposes, consulting with a healthcare professional is always a wise and responsible step.
cannabis consumption

Cannabis has incredible potential, and when approached with respect, knowledge, and self-awareness, it can enrich our lives. By engaging in this honest conversation, we strengthen our community and foster a culture of mindful, healthy cannabis consumption for everyone.

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The Art of Intentional Cultivation

Intentional cultivation

Every successful cannabis harvest begins long before a seed sprouts. It starts with a clear vision: what do you want your finished product to achieve? The remarkable versatility of the Cannabis sativa plant means it can be cultivated for a myriad of purposes – from the connoisseur’s prized flower to the high-potency material for extracts, the functional base for edibles, or simply the sheer joy of nurturing a living plant.

Gone are the days when a single growing approach sufficed for every ambition. To truly unlock the plant’s potential and meet your specific goals, your cultivation strategy – from strain selection to post-harvest processing – must be meticulously aligned with your ultimate application. Let’s delve into how different end goals shape your decisions in the grow space, informed by the latest scientific understanding of cannabis chemistry and breeding.

Seedsman - Cannabis Super Store

The Foundation of Purpose: Understanding Cannabis “Chemovars”

Traditional cannabis labels like “Indica,” “Sativa,” and “Hybrid” offer a general idea of plant structure or a strain’s lineage, but they fall short in predicting specific effects. Modern understanding points to the chemical profile as the true driver of experience. Scientists often use the term “chemovar” (chemical variety) to classify cannabis based on its unique blend of compounds, particularly:

  1. Cannabinoids: The most famous compounds, like THC (for psychoactivity) and CBD (for potential therapeutic benefits). But also the vast array of minor cannabinoids (e.g., CBG, CBN, THCV) that contribute to the plant’s complex effects and are gaining significant research interest, as highlighted in studies on topical cannabis applications. The ratio and total concentration of these compounds in a strain are paramount.
  2. Terpenes: These aromatic molecules are responsible for the distinct scents and flavours of cannabis – think pine, citrus, earth, or sweet notes. Beyond aroma, terpenes are pharmacologically active, possessing properties like anti-inflammatory, analgesic, or sedative effects. Crucially, they interact with cannabinoids to create the “entourage effect,” a synergy that can profoundly shape the overall experience. Research using advanced techniques like headspace GC-MS now provides a deeper insight into these often-subtle aromatic undertones and their correlations with cannabinoid profiles.

Historically, cannabis breeders selected strains based on desirable aromas, inadvertently shaping their terpene biosynthesis. Just as master brewers select yeast strains for specific flavours and fermentation profiles, cannabis cultivators increasingly select specific chemovars for their precise chemical output. We wrote about this last week and in-depth in the past. Check out those blogs here.

Tailoring Your Grow: Strategies for Different Applications

Once you define your end goal, your cultivation pathway becomes clearer:

1. Growing for Top-Tier Flower (Recreational & Medicinal Enjoyment)

When the goal is premium flower for smoking or vaporising, “bag appeal” (visual aesthetics) and “terpene expression” are paramount.

  • Strain Selection:
    • Terpene Prowess: Prioritise strains known for rich, complex terpene profiles. These are what deliver the nuanced aromas, distinct flavours, and contribute significantly to the overall experience through the entourage effect. Look for genetics that consistently express high levels of desirable terpenes.
    • Desired Effects: Choose chemovars with cannabinoid ratios and terpene profiles that align with the intended experience—be it uplifting and creative (e.g., high Limonene), deeply relaxing (e.g., high Myrcene), or balanced for a functional effect.
    • Aesthetics & Structure: Select strains genetically predisposed to dense bud structure, vibrant colours, and abundant trichome coverage, as these contribute directly to visual appeal and resin content.
  • Cultivation & Post-Harvest:
    • Precise Environmental Control: Maintaining optimal temperature, humidity, and airflow throughout the flowering stage is critical for maximising terpene production and preventing mould. This is an ongoing battle, especially in variable climates.
    • Peak Harvest Timing: Harvest at the precise moment when trichomes indicate peak maturity for desired cannabinoids and terpenes (e.g., majority cloudy with some amber), as this window maximises the target profile.
    • Meticulous Drying & Curing: As previously discussed, proper drying and slow curing are non-negotiable for preserving delicate terpenes, preventing degradation of cannabinoids, and developing a smooth, flavorful final product. Burping jars correctly or utilising humidity control packs is essential for optimal curing.
intentional cultivation

2. Growing for Concentrates (Extracts)

Cultivating for extracts shifts the focus from the plant’s physical form to maximising the quantity and quality of its resin (trichomes), which will be separated and refined into products like rosin, shatter, wax, or diamonds.

  • Strain Selection:
    • Trichome Abundance & Quality: The primary criteria here are strains known for producing copious amounts of large, easily rupturable trichomes. Some genetics are simply better “resin producers.”
    • Target Cannabinoid/Terpene Concentration: Different extracts might favour specific cannabinoid profiles (e.g., high THCA for diamonds, diverse cannabinoids for full-spectrum extracts). Terpene concentration within the trichomes is also crucial, especially for “live” extracts that prioritize fresh aroma.
    • Extraction Efficiency: Consider how well the trichomes detach from the plant material, which can vary by strain and influence yield with methods like ice water extraction or dry sifting.
  • Cultivation & Post-Harvest:
    • Cleanliness: Maintaining an impeccably clean grow environment is paramount. Any contaminants (dust, pest residues) on the plant will end up in the extract, compromising purity.
    • Harvest Timing: For “live” extracts (like live rosin or live resin), plants are often harvested slightly earlier than for smoking flower and immediately flash-frozen to preserve volatile terpenes at their freshest. For dry extracts, efficient drying of resinous material is key.
    • Targeted Drying: If drying for extracts, methods prioritise rapid moisture removal from the trichomes while preserving them, rather than the slow, nuanced cure of smoking flower.

3. Growing for Edibles

Cultivating for edibles prioritises total cannabinoid yield that can be efficiently infused into food products. While terpenes can contribute to flavour, they are often secondary to raw cannabinoid quantity.

  • Strain Selection:
    • Overall Cannabinoid Mass: Focus on strains known for high cannabinoid production throughout the entire plant, including trim and smaller buds, not just the large top colas.
    • Desired Cannabinoid: Whether you want THC-dominant or CBD-dominant edibles will guide your choice.
    • Ease of Decarboxylation: While most cannabis will decarboxylate with heat, selecting strains that convert efficiently is beneficial.
  • Cultivation & Post-Harvest:
    • Full Plant Utilisation: This goal allows for efficient use of a wider range of plant material – not just premium buds, but sugar leaves and smaller nugs – all of which contain valuable cannabinoids.
    • Decarboxylation: A controlled heating process is crucial to convert the raw, acidic cannabinoids (THCA, CBDA) into their active, non-acidic forms (THC, CBD) for maximum effect in edibles. This can be done post-drying or as a separate step before infusion.
    • Batch Consistency: For commercial edible production, consistent cannabinoid content across batches is essential, requiring precise measurement and processing protocols.
intentional cultivation

4. Growing for the Love of It (Hobbyist/Learning)

For many home growers, the primary motivation is the intrinsic reward of cultivation itself—the joy of watching a plant grow, the learning process, and the satisfaction of producing their own cannabis, regardless of commercial metrics.

  • Strain Selection:
    • Curiosity & Preference: Choose strains based on appealing aromas, unique growth characteristics, or simply the desire to try something new.
    • Ease of Growth: For beginners, selecting robust and forgiving strains allows for a smoother learning curve, building confidence without the frustration of finicky genetics.
    • Novelty & Experimentation: This goal encourages exploring unusual chemovars or trying experimental techniques without the pressure of a bottom line.
  • Cultivation & Post-Harvest:
    • Emphasis on Learning: Every grow is an opportunity to experiment with different techniques (e.g., nutrient adjustments, training methods) and observe plant responses firsthand.
  • Process Over Perfection: While striving for quality, the focus remains on enjoying the journey of cultivation, understanding the plant’s life cycle, and the satisfaction of producing your own cannabis, regardless of achieving peak commercial yield or potency.
Seedsman - Cannabis Super Store
Seedsman - Cannabis Super Store

Your Compass for the Cannabis Garden

Defining your cannabis cultivation goals upfront is the most powerful tool in your grower’s arsenal. By understanding the distinct demands of each application – from the precise chemical profiles for top-tier flower or extracts to the efficient cannabinoid yields for edibles, or the sheer joy of hobbyist growing – you can make informed decisions from strain selection to final product. Leverage lab testing to understand the true chemical signature of your chosen genetics, and embrace intentional cultivation to achieve consistently rewarding results.

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Your Cannabis Compass: Navigating Strains Beyond Indica and Sativa

indica and sativa
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Stepping into the world of cannabis can be an exciting adventure, but it’s also one filled with choices. Rows of jars display strains with intriguing names, each promising a unique experience. For many, the initial instinct is to ask, “Is this Indica, Sativa, or Hybrid?” While these categories are a common starting point, they represent just one piece of a much larger, more fascinating puzzle.

The truth is, predicting your cannabis experience is far more personal and complex than relying solely on the plant’s physical structure or traditional classifications. Scientific research increasingly shows that the effects you feel are driven primarily by the plant’s unique chemical profile and how that profile interacts with you, your body, your mind, and your environment.

Let’s go beyond the labels and explore the factors that truly influence your cannabis journey, giving you the tools to make more informed and satisfying choices.

The Chemical Signature: Cannabinoids, Terpenes, and the Entourage Effect

Think of each cannabis strain as having a distinct chemical signature, defined mainly by two key groups of compounds found in its resinous trichomes:

  1. Cannabinoids: These are the headline compounds cannabis is most known for.
    • THC (Tetrahydrocannabinol): The primary psychoactive compound, responsible for the euphoric or “high” sensation. Its concentration is often a major factor in a strain’s perceived potency.
    • CBD (Cannabidiol): Non-intoxicating, CBD is widely researched for its potential therapeutic properties, often associated with calming, anti-inflammatory, and pain-relieving effects.
    • Minor Cannabinoids: Beyond THC and CBD, over a hundred other cannabinoids exist, like CBG (Cannabigerol) and CBN (Cannabinol). Research is ongoing to understand their specific roles and potential benefits, including areas like appetite stimulation or anti-inflammatory action, as explored in studies on topical cannabis applications for skin conditions. The specific ratio and total concentration of all these cannabinoids in a strain significantly shape its overall impact.
  2. Terpenes: These fragrant molecules give cannabis its diverse aromas and flavours – from citrusy notes to earthy, piney, or sweet profiles. But terpenes do more than just smell good; they are also believed to interact synergistically with cannabinoids and your body’s own endocannabinoid system. This interaction, known as the entourage effect, suggests that the specific combination of terpenes and cannabinoids working together can modulate and enhance the overall effects, creating a more nuanced experience than any single compound could provide alone.
indica and sativa

The “Indica vs. Sativa vs. Hybrid” labels often loosely correlate with certain cannabinoid and terpene profiles or growth patterns, but they are not definitive predictors of effect for every individual or every batch of a strain. Focusing on the actual chemical profile is more accurate. I have written about Chemovars in the past and how the lingo of Terpenese has taken over some parts of the world to the point that you won’t find strain names on the products but simply their chemovar expressions.

The Human Equation: You, Your Mindset, and Your Surroundings

Your personal cannabis experience isn’t just about the plant’s chemistry; it’s a dynamic interaction involving you and your environment:

  1. Your Unique Physiology: Your body’s endocannabinoid system, genetics, metabolism, past cannabis use, and overall health all play a role in how you react to cannabinoids and terpenes. What provides deep relaxation for one person might be stimulating for another.
  2. Your Intent (Why): What is your goal for this cannabis experience? Are you seeking relief from discomfort (like pain or anxiety), looking for creative inspiration, hoping to unwind and relax, or preparing for sleep? Your purpose helps guide your selection.
  3. Your Set (Mindset): Your current mood, expectations, and psychological state are powerful influencers. Approaching cannabis with a positive mindset in a comfortable state can enhance desired effects, while stress or anxiety might amplify potential negative reactions, especially with higher THC strains.
  4. Your Setting (Environment): Where and with whom are you consuming? The same strain enjoyed alone at home might feel very different at a concert or a social gathering. Your physical and social surroundings shape the experience.
indica and sativa

Understanding that your experience is a blend of the plant’s chemistry and these personal factors is key to making a more mindful choice. Once you actually think about these things… More of those random anxiety attacks after the weed might make more sense. It’s important to have an open conversation with your Budtender, too. Recommendations can truly make or break your experience, for budtenders, knowing what state of mind, the intent and vibes a consumer wants to achieve helps so much more.

Navigating the Options: A Mindful Selection Process

Moving beyond rigid categories requires a different approach. Use these steps to guide your selection:

  1. Define Your Intent: Start by clearly identifying why you are using cannabis. Is it for recreational enjoyment, creative pursuits, or potential therapeutic benefits (like managing discomfort, anxiety, or improving sleep)?
  2. Consider Your Set and Setting: Think about your current mood and where you’ll be. Are you in a relaxed environment where you can comfortably unwind, or do you need to remain functional and engaged?
  3. Explore the Chemical Profile: This is where you become a detective.
    • Check Lab Results: Don’t guess the potency or profile. Use the lab testing results provided by reputable dispensaries. These reports show the percentages of major cannabinoids (THC, CBD) and often list the dominant terpenes. This is your most reliable source of information about the actual chemical makeup of the product you’re considering. Lab testing is also crucial for ensuring the product is free from harmful contaminants, linking back to the importance of purity in cultivation and processing. Something that was once not even cost-effective can now be achieved by home growers too. 
    • Look at Cannabinoid Ratios: Are you interested in high THC for psychoactivity, balanced THC/CBD for a more modulated experience, or high CBD for therapeutic focus without strong intoxication? As strains become more studied and bred for more complexity in flavour profiles and effects, understanding the ratio of cannabinoids will become key for most medical applications.
  4. Investigate Terpene Profiles: Do the aromas and potential effects associated with those terpenes (like Myrcene for relaxation, Limonene for uplift, or Caryophyllene for anti-inflammatory potential, drawing from our skin health discussion) align with your Intent and Set?
indica and sativa
  • Know Your Tolerance: If you’re new to cannabis or a specific strain, start with a very low dose and go slow. This is the best way to gauge your individual reaction and avoid an uncomfortable experience.
  • Factor in Cultivation Nuances: While not something you need to obsess over, understand that environmental factors during growth (like nutrients or stress, as discussed in research) can subtly influence the final cannabinoid and terpene profile, making each batch unique. This is another reason why lab testing is so valuable – it tells you the profile of this specific product.
  • Consult the Experts: Don’t hesitate to talk to the knowledgeable staff at your dispensary, weed shop or Rasta. Share your Intent, Set & Setting, tolerance, and desired profile. They can offer personalised recommendations based on their product knowledge, the available lab data, and feedback from other consumers.

Managing Expectations: Science vs. Story

It’s important to approach strain selection with realistic expectations. While user reports and anecdotal evidence abound, scientific research is still catching up to definitively confirm the specific effects of individual strains or the precise medical benefits attributed to every cannabinoid and terpene combination. Studies, from preclinical lab work to clinical trials (like those exploring cannabis for skin conditions), are ongoing to build this evidence base. Use anecdotal information as interesting possibilities, but rely on the chemical profile and your own experience to guide you.

The Personal Journey of Discovery

Ultimately, choosing the right cannabis strain is a personal journey of discovery. It’s about moving beyond potentially misleading labels and becoming a more informed consumer who understands the interplay between plant chemistry, individual biology, mindset, and environment.

indica and sativa

By focusing on your Intent, being aware of your Set and Setting, utilising Lab Testing to understand the true Chemical Profile (Cannabinoids and Terpenes), and consulting with dispensary experts, you gain a powerful compass to navigate the vast landscape of cannabis strains. Embrace the process, start low and slow, and enjoy finding the unique combinations that create the perfect experience for you.

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Cannabis Nutrition in the Vegetative Stage: A New Study

Cannabis Nutrition

For cultivators, navigating cannabis nutrition can feel like a constant quest for the perfect recipe. Unlike established agricultural crops with decades of dedicated research, the science of cannabis cultivation is still rapidly evolving, driven in part by recent legalisation and increasing commercial interest in this high-value plant. Finding precise, data-backed guidance on feeding schedules and nutrient ratios remains a key challenge for growers aiming to maximise both yield and quality.

This is precisely why studies like the recent publication by Kpai et al. (2024) in Frontiers in Plant Science are so valuable. They took a rigorous, scientific approach to evaluate the mineral requirements of cannabis during the critical vegetative stage, focusing on the interplay between the three primary macronutrients: Nitrogen (N), Phosphorus (P), and Potassium (K). Let’s unpack this research to gain a more in-depth understanding of its findings and how they might inform your cultivation strategy.

cannabis nutrition

The Research at a Glance: What, How, and Why It Matters

The Focus: The study specifically investigated the impact of different combinations and concentrations of N, P, and K on the vegetative growth of a particular medical cannabis strain, “The New,” grown in a Deep-Water Culture (DWC) hydroponic system. The vegetative phase is when cannabis plants build their structural framework – developing strong stems, a robust root system, and ample foliage – all essential for supporting vigorous flowering and heavy buds later on.

The Method: To move beyond simply testing nutrients one at a time, the researchers employed Response Surface Methodology (RSM). This advanced statistical technique allowed them to evaluate how N, P, and K interacted with each other across a range of concentrations simultaneously. In a DWC system, plant roots are suspended directly in the nutrient solution, providing a controlled environment ideal for precisely manipulating nutrient levels and observing direct plant responses. They grew clones of the cannabis strain in these DWC units for a two-week vegetative period.

Why it Matters: Because the medical and adult-use cannabis market is a high-stakes industry, optimising every stage of growth is crucial for profitability and consistency. So many of us don’t want to admit this. But yes, it is a commercial product at scale. Understanding the nuanced nutritional needs of the plant, including how different key nutrients interact, helps growers use fertilisers more efficiently, potentially reducing costs and minimising environmental impact from nutrient runoff – a significant concern in many agricultural regions. Personally, I am an organic grower. But the reality is that on large-scale commercial farms, things are done differently. Research like this makes a difference to those farmers on scale who care but are still bound by traditional methods. I recognise that not everyone can just make a flip from chemical-based fertilisers to full organic integrations. The amount of food produced will significantly drop, which will not be good. But I digress.

cannabis nutrition

Peeling Back the Layers: What They Measured

To get a comprehensive picture of plant health and growth under varying nutrient conditions, the researchers measured a wide array of parameters throughout the study:

  • Physical Growth Metrics: This included plant height, width, stem diameter, number of branches, and number of leaves. They also calculated a Growth Index (combining height and width) for an overall measure of size.
  • Biomass Accumulation: They carefully weighed the fresh and dry mass of leaves, stems, and roots to understand how nutrients influenced the total amount of plant material produced.
  • Leaf Function: Leaf area (total surface for photosynthesis) and specific leaf area (ratio of leaf area to dry leaf mass) provided insights into the plant’s photosynthetic capacity and resource allocation.
  • Chlorophyll Content: Measured for both chlorophyll a and b, this directly indicates the plant’s ability to capture light energy for growth.
  • Nutrient Use Efficiency: This metric is vital from a sustainability perspective, showing how effectively the plant converts the supplied N, P, and K into biomass.
  • Leaf Tissue Elemental Analysis: Critically, they didn’t just look at what was in the solution but also what nutrients were taken up and present in the plant leaves. This provides a benchmark of optimal internal nutrient levels associated with healthy vegetative growth.
cannabis nutrition

Key Discoveries: The Science Behind the Feed

The study yielded several significant findings, offering deeper insights into cannabis vegetative nutrition in hydroponics:

Nutrient Interactions Rule Vegetative Growth: The RSM approach powerfully demonstrated that the interactions between N, P, and K were far more influential than the effects of individual nutrients in isolation for most vegetative growth parameters. The interplay between N x K, K x P, and the combined N x P x K significantly affected everything from root and stem mass and diameter to leaf area, chlorophyll content, and nutrient use efficiencies. This confirms that achieving optimal vegetative growth isn’t about hitting specific high numbers for each nutrient individually, but about finding the right balance in the solution.

Optimal Nutrient Levels Within the Leaves Identified: By analysing the plant tissues, the researchers could pinpoint the target internal nutrient concentrations associated with desirable growth. For the specific cannabis accession used, the optimal leaf tissue levels were found to be:

    • Total Nitrogen (TN): 0.54 mg g⁻¹
    • Phosphorus (P): 0.073 mg g⁻¹
    • Potassium (K): 0.27 mg g⁻¹
    • Calcium (Ca): 0.56 mg g⁻¹
    • Sulfur (S): 0.38 mg g⁻¹

Monitoring leaf tissue levels and comparing them to these benchmarks could be a valuable diagnostic tool for growers to ensure their plants are effectively taking up and utilising nutrients. The study also noted an interesting observation: Phosphorus seemed to be stored in the leaves rather than immediately used for vegetative growth in this phase, potentially explaining why lower P levels in the solution were found to be optimal for the vegetative stage.

cannabis nutrition

P and K Can Impact Magnesium Uptake: The study found a clear interaction where increasing concentrations of Phosphorus and Potassium in the nutrient solution led to a decrease in the Magnesium (Mg) concentration within the leaves. Nitrogen levels, however, did not show this antagonistic effect on Mg. This highlights a specific nutrient competition dynamic that growers should be aware of, as inadequate Mg can lead to deficiencies visible as yellowing between leaf veins. Ensuring sufficient, but not excessive, P and K relative to Mg is important.

Data-Backed Nutrient Solution Recommendations for Vegetative Hydro: Synthesising their findings across all measured parameters, and focusing on maximising both desired growth and nutrient use efficiency, the study provided specific nutrient solution recommendations for Cannabis sativa in the vegetative stage within a hydroponic system:

    • Nitrogen (N): 160–200 mg L⁻¹
    • Phosphorus (P): 30 mg L⁻¹
    • Potassium (K): 60 mg L⁻¹

These levels represent the researchers’ data-driven optimal range based on their experimental conditions and the specific cultivar studied.

Implications for Your Grow

These findings offer practical insights, particularly for hydroponic growers in the vegetative phase:

  • Rethinking Phosphorus: The recommended Phosphorus level of 30 mg L⁻¹ is notably lower than what is sometimes recommended in general cannabis feeding charts or philosophies that may push higher P even in veg to “prepare” for flower. This study suggests that for vegetative growth specifically, lower P levels are optimal when N and K are balanced, and excess P might not translate to better growth, potentially just being stored.
  • Balance Over Broad Strokes: Forget chasing excessively high numbers for individual nutrients. This research emphasises that the ratio and interaction between N, P, and K are paramount. Using a balanced approach like the recommended 160-200 N, 30 P, 60 K could unlock better vegetative performance and efficiency.
  • Efficiency and Sustainability: Adopting nutrient levels closer to these scientifically backed optimums means less wasted fertiliser. This translates directly into cost savings and reduced environmental impact, aligning with more sustainable cultivation practices.
  • Adaptation is Key: While this study provides a powerful benchmark, remember it was conducted with a specific cannabis accession in a DWC system. Different cultivars may have slightly varied needs, and other growing methods (like coco coir, soil, or aeroponics) have different nutrient dynamics. Use these recommendations as an educated, science-based starting point for your hydroponic vegetative feed, and continue to monitor your plants closely, making adjustments based on their specific responses. Observing leaf tissue nutrient levels could also be a valuable tool.

Our Final Take

The Kpai et al. (2024) study is an excellent example of the kind of rigorous, data-driven research that is elevating cannabis cultivation from art to science. By employing advanced techniques like RSM, they’ve provided clear evidence of complex nutrient interactions and offered specific, actionable recommendations for vegetative-stage hydroponic feeding. The insights into lower optimal P levels and the P/K-Mg interaction are particularly noteworthy.

This research is a critical piece in the larger puzzle of optimising cannabis nutrition across all stages and systems. As more studies like this emerge, growers will have increasingly precise tools to achieve maximum yield, quality, and sustainability. If you’re currently in the vegetative phase in hydroponics, considering the 160-200 N, 30 P, 60 K recommendation from this study is a great way to leverage cutting-edge science in your cultivation practices.

What are your thoughts on these findings? Will you be adjusting your vegetative feed based on this research?

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Can You Grow Cannabis Through Winter?

Can you Grow Cannabis through winter

The harvest is in, the trim scissors are (momentarily) at rest, and as the days grow shorter and colder, many growers find themselves contemplating the next cycle. Could this winter be the time to keep the cultivation going? Is growing cannabis possible when the temperatures drop and the sunlight dwindles?

Growing cannabis in cold weather certainly presents unique challenges compared to the warm, sunny months. However, with the right knowledge, preparation, and a bit of ingenuity, it is absolutely possible to achieve a successful winter grow. This post will explore how cold affects your plants, what you need to consider before committing to a winter cycle, and how to maximise your chances of success.

can you grow cannabis through winter

Cannabis and the Cold: What Happens When Temperatures Drop?

Cannabis, a plant that thrives in warmer climates, reacts significantly when exposed to cold. It’s not built for freezing temperatures, and prolonged or intense cold can stress and damage your plants in several ways:

  • Slowed Metabolism and Growth: Just like many biological processes, the plant’s metabolic rate slows down in cold. This leads to significantly slower growth and overall poorer plant development, impacting everything from vegetative growth to flower formation.
  • Reduced Photosynthesis: Cooler temperatures reduce the plant’s ability to photosynthesise efficiently. With less energy production, the plant struggles to develop properly and produce robust flowers.
  • Water and Nutrient Uptake Issues: Cold soil can make it difficult for roots to absorb water and nutrients. This can lead to nutrient deficiencies, even if the nutrients are present in the soil, as the plant can’t access them effectively.
  • Increased Stress and Vulnerability: Cold itself is a major stressor. Stressed plants are generally weaker and more susceptible to other problems, particularly diseases and pests.
  • Risk of Mould and Disease: Cold and wet conditions, often associated with winter, create an ideal environment for fungi and other pathogens. This significantly increases the risk of root rot, powdery mildew, and other devastating diseases.
  • Impact on Cannabinoids and Terpenes: While some studies suggest cold can influence the plant’s chemical composition, potentially leading to the formation of unique terpenes and cannabinoids, others indicate that significant cold stress or cold acclimation periods might actually decrease total cannabinoid content and overall yield. The relationship is complex, but it’s clear cold can alter the final product’s profile.

Winter Growing: Outdoors vs. Indoors

Given the adverse conditions, the first major decision is where to grow.

Growing Outdoors in Winter:

In most parts of South Africa, you can attempt a winter grow. Some areas will be wetter, like the Western Cape, with less sun exposure, while in regions with true freezing winters like the Free State, cultivating cannabis outdoors without significant environmental control is incredibly difficult, if not impossible, for most varieties. So, depending nowhere you live straight away this will or won’t be an option for you outdoors. While outdoor growing during warmer months offers advantages like ample natural sunlight and space, the disadvantages in winter are often overwhelming:

  • Unpredictable Temperatures: Freezing or chilly nights and fluctuating temperatures are major hurdles, potentially causing severe plant damage or death. Growers will have to put in the effort of moving plants to more favourable areas in the evening times too. 
  • Limited Sunlight: Short days and often cloudy conditions mean significantly less natural light, requiring supplemental lighting or resulting in stunted growth and low yields. Without supplementary light, most winter crops will give very weak plants, as the sunlight might not be intense enough. In places like Limpopo and the Karoo, Sunlight in winter is not a problem really. 
  • Increased Vulnerability: The cold weakens plants, making them easier targets for opportunistic pests and diseases that might survive the cold. For those who are serious about a winter grow, they will be supplementing plants with various nutrients and minerals to help with these vulnerabilities. Grower knowledge is key here.
  • Restricted Growth Cycle: The natural season is cut short, limiting the plant’s time to grow and flower. This is normal and should be accepted for the winter months, unless you have a commercial setup. Grow 3 plants extra to supplement your needs than expect more from the winter crop.
can you grow cannabis through winter

Growing Indoors in Winter:

For most growers in colder climates, moving indoors is the most practical and reliable approach for winter cultivation. While it requires an initial investment in equipment, it offers crucial advantages:

  • Controlled Environment: Indoors, you have complete control over temperature, humidity, lighting, and ventilation – the critical factors for healthy cannabis growth. This eliminates the unpredictability of outdoor winter weather.
  • Year-Round Growing: With a controlled environment, you can run cycles regardless of the outdoor season, maximizing your production.
  • Reduced Risk of Pests and Disease: While not immune, indoor environments are generally easier to keep clean and free from the widespread pest and disease pressures found outdoors, especially in less-than-ideal winter conditions.

While most won’t want to invest in this, consider perhaps just one half of the garage for extra light and warmth on cold and wet days, as well as in the evenings. When the plants hit flower, winter will be on its tail end, and you will be able to keep them outside. But be mindful, every province has unique weather during winter and summer. Some in KZN grow much better in winter, just due to the temperature and overall drop in humidity. While people in Maggaliesburg are growing year round no problem. Or in Nelspruit, where it rains every time at the right time.

can you grow cannabis through winter

Key Considerations Before Committing to a Winter Grow

Whether you attempt a heavily protected outdoor setup or transition indoors, successful winter cultivation demands careful planning and management. Here are the critical factors:

  • Variety Selection: This is paramount. Choose varieties known for their resilience to cold temperatures and resistance to mould and disease. Varieties with shorter flowering periods are also advantageous, as they finish faster, reducing the window of potential exposure to harsh conditions or the need for extremely long indoor cycles. Look for genetics specifically bred for climate resistance.
  • Environment and Soil Preparation: Proper preparation is non-negotiable.
    • Drainage: Cold, wet conditions can lead to waterlogged soil, a major killer of roots and a breeding ground for fungi. Ensure your pots or beds have excellent drainage. Adding materials like perlite or vermiculite to your soil mix can significantly help.
    • Nutrients: Colder soil slows down the breakdown of organic matter, meaning nutrients are released more slowly. Using high-quality organic fertilizers and potentially supplementing with readily available nutrients like phosphorus and potassium (crucial for root and flower development) can help ensure your plants get what they need.
    • Soil Temperature & pH: Aim to keep your soil temperature within a suitable range (avoiding freezing). Monitoring soil pH is also vital (6-7 is ideal for cannabis) to ensure efficient nutrient uptake, which is already challenged by the cold.
  • Temperature Control: Maintaining the right air and soil temperature is essential. Aim for daytime temperatures between 18-26°C and slightly cooler nights (10-18°C) to mimic natural conditions without causing stress. Indoor growers will need reliable heating. Outdoor or greenhouse growers will need heating and possibly thermal insulation (blankets, poly sheeting) to protect against frost and temperature swings.
  • Lighting: With significantly reduced natural light, especially for indoor grows, adequate artificial lighting is necessary. Understanding your plant’s light needs (vegetative vs. flowering) and using timers to provide the correct photoperiod is crucial for successful development.
  • Watering: Cold conditions mean less evaporation from the soil. It is critical to water less frequently than you would in warmer months. Always check soil moisture before watering to avoid overwatering and waterlogging. Watering in the morning or afternoon allows excess moisture to evaporate before cooler nights settle in.
can you grow cannabis through winter

Winter Yields and What to Expect

While challenging, a successful winter grow can yield quality cannabis. Be prepared that yields might differ from your peak summer or perfectly controlled indoor cycles, especially if dealing with less-than-ideal conditions or cold stress. The final cannabinoid and terpene profiles could also be influenced by the colder environment, potentially resulting in unique characteristics, though possibly lower overall concentrations depending on the severity and timing of cold exposure.

The cannabis you cultivate this winter will still require the crucial post-harvest steps we’ve discussed, like drying and curing, to fully develop its flavour, aroma, and smoothness. And depending on the quality and type of product you yield, it could be destined for the world of extracts, transforming into potent and versatile concentrates.

Growing cannabis through winter is a demanding but rewarding endeavour. It requires meticulous planning, careful environmental control, and diligent plant care. By selecting the right varieties, preparing your growing space properly, and managing temperature, light, and watering effectively, you can successfully navigate the cold weather challenge. Consider the factors discussed, weigh the pros and cons of indoor versus outdoor, and decide if you’re ready to embrace the winter grow cycle.

With the right approach, impressive results are achievable, allowing you to continue harvesting your own quality cannabis, even when the world outside is facing the freeze.